Consortium PSYCHIATRICUMConsortium PSYCHIATRICUMConsortium PSYCHIATRICUM2712-76722713-2919Eco-Vector17110.17816/CP171RESEARCHИССЛЕДОВАНИЕResearch ArticleTelomere Length as a Marker of Suicidal Risk in SchizophreniaДлина теломер как маркер суицидального риска при шизофренииhttps://orcid.org/0000-0001-7507-327XZakharovaNatalia V.ЗахароваНаталья Вячеславовна
Russian Federation

PhD, MD, Head of the Laboratory for Fundamental Research Methods of the Scientific Research Center of Neuropsychiatry

руководитель Лаборатории фундаментальных методов исследования НКИЦ нейропсихиатрии, кандидат медицинских наук

nataliza80@gmail.comhttps://orcid.org/0000-0001-5380-4406BravveLidia V.БраввеЛидия Викторовна
Russian Federation

MD, psychiatrist, junior researcher of the Laboratory for Fundamental Research Methods of the Scientific Research Center of Neuropsychiatry

bravve95@yandex.ruhttps://orcid.org/0000-0003-4481-7840MamedovaGalina Sh.МамедоваГалина Шакировна
Russian Federation

Clinical resident, assistant of the Laboratory for Fundamental Research Methods of the Scientific Research Center of Neuropsychiatry

gsh.mamedova@yandex.ruhttps://orcid.org/0000-0002-1516-082XKaydanMaria A.КайданМария Андреевна
Russian Federation

MD, psychiatrist, junior researcher of the Laboratory for Fundamental Research Methods of the Scientific Research Center of Neuropsychiatry

kaydan.maria@yandex.ruhttps://orcid.org/0000-0003-1206-5832ErshovaElisaveta S.ЕршоваЕлизавета Сергеевна
Russian Federation

PhD in Biology, leader scientific researcher of the Laboratory of Molecular Biology, Department of Molecular Biology

es-ershova@rambler.ruMartynovAndrey V.МартыновАндрей Владимирович
Russian Federation

PhD in Biology, senior scientific researcher of the Laboratory of Molecular Biology, Department of Molecular Biology

avlamar@mail.ruVeikoNatalia N.ВейкоНаталья Николаевна
Russian Federation

PhD in Biology, chief scientific researcher of the Laboratory of Molecular Biology, Department of Molecular Biology

satelit32006@yandex.ruKostyukSvetlana V.КостюкСветлана Викторовна
Russian Federation

PhD in Biology, Head of the Laboratory of Molecular Biology, Department of Molecular Biology

svet-vk@yandex.ruMental Health Сlinic No.1 named after N.A. AlexeevГБУЗ «Психиатрическая клиническая больница № 1 им. Н.А. Алексеева Департамента здравоохранения города Москвы»Research Center for Medical GeneticsФГБНУ «Медико-генетический научный центр им. Н.П. Бочкова»21062022050720223237472903202230052022Copyright ©; 2022, Zakharova N.V., Bravve L.V., Mamedova G.S., Kaydan M.A., Ershova E.S., Martynov A.V., Veiko N.N., Kostyuk S.V.Copyright ©; 2022, Захарова Н.В., Бравве Л.В., Мамедова Г.Ш., Кайдан М.А., Ершова Е.С., Мартынов А.В., Вейко Н.Н., Костюк С.В.2022Zakharova N.V., Bravve L.V., Mamedova G.S., Kaydan M.A., Ershova E.S., Martynov A.V., Veiko N.N., Kostyuk S.V.Захарова Н.В., Бравве Л.В., Мамедова Г.Ш., Кайдан М.А., Ершова Е.С., Мартынов А.В., Вейко Н.Н., Костюк С.В.https://creativecommons.org/licenses/by/4.0https://consortium-psy.com/jour/article/view/171

BACKGROUND: Schizophrenia and suicidal behavior are associated with shortening in the length of telomeres. The aim of the study was to compare the content (pg/mcg) of telomeric repeat in DNA isolated from peripheral blood cells in three groups of subjects: patients with schizophrenia and a history of suicide attempts, patients with schizophrenia without suicidal tendencies, and healthy control volunteers.

METHODS: Relapses according to gender and age were examined in 47 patients with schizophrenia with suicidal behavior, 47 patients without self-destructive conditions, and 47 volunteers with healthy control and maintenance for the content of telomeric and the number of copies of mitochondrial DNA (mtDNA) in peripheral blood leukocytes.

RESULTS: Analysis of determining the content of telomeric repeat (TR) in the DNA of massive weight gain in the series: patients with schizophrenia and suicidal attempts — patients with schizophrenia without suicidal observations — healthy controls (225±28.4 (227 [190; 250]) vs. 243±21 (245 [228; 260]) vs. 255±17.9 (255 [242; 266]), p <0.005. The same trend is observed for the number of mtDNA copies (257±101.5 (250 [194; 297])) vs. 262.3±59.3 (254 [217; 312]) vs. 272±79.9 (274 [213; 304]); p=0.012), but no significant differences were recorded.

CONCLUSIONS: For the first time, the phenomenon of telomere shortening was discovered in schizophrenics with suicidal risk. The length of the telomere corresponds to the parameter of a biological marker — an objectively measured indicator of normal or pathological processes, but gaining an idea of its reliability is still necessary for verification with an assessment of its sensitivity, specificity, and positive and negative predictive value. The telomere may be considered a putative predictive indicator of suicidal risk.

ВВЕДЕНИЕ: Шизофрения и суицидальное поведение сопряжены с укорочением длины теломер. Целью исследования стало сравнение содержания (пг/мкг) теломерного повтора в ДНК, выделенной из клеток периферической крови, у трех групп испытуемых: пациентов с шизофренией и суицидами в анамнезе, пациентов с шизофренией без суицидов и добровольцев из числа здорового контроля.

МЕТОДЫ: Обследованы сопоставимые по полу и возрасту 47 пациентов с шизофренией с суицидальным поведением, 47 больных без аутодеструктивных тенденций и 47 добровольцев здорового контроля клинически и на предмет содержания теломерного повтора в ДНК лейкоцитов крови.

РЕЗУЛЬТАТЫ: Анализ определения содержания теломерного повтора (TR) в ДНК свидетельствует о статистически значимом увеличении длины теломер в ряду: пациенты с шизофренией и суицидами — пациенты с шизофренией без суицидов — здоровый контроль (225 vs 224 vs 255; p=0,024). Такой же тренд прослеживается для концентрации митохондриальной ДНК (мтДНК) (257 vs 262 vs 272; p=0,012).

ВЫВОДЫ: Впервые установили феномен укорочения длины теломер при шизофрении, сопряженной с суицидальным риском. Длина теломер отвечает параметрам биологического маркера — объективно измеряемого показателя нормальных или патологических процессов, но его валидность еще необходимо проверить с оценкой его чувствительности, специфичности, положительной и отрицательной прогностической ценности. Рассматривать длину теломер как прогностический индикатор суицидального риска преждевременно.

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